Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 403530
Title Phase toxicity of dodecane on the microalga Dunaliella salina
Author(s) Kleinegris, D.M.M.; Es, M. van; Janssen, M.G.J.; Brandenburg, W.A.; Wijffels, R.H.
Source Journal of Applied Phycology 23 (2011)6. - ISSN 0921-8971 - p. 949 - 958.
DOI http://dx.doi.org/10.1007/s10811-010-9615-6
Department(s) Bioprocess Engineering
CVI Diagnostics and Crisis
PPO/PRI AGRO Multifunctioneel Landgebruik
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) beta-carotene production - 2-phase bioreactors - extraction - system
Abstract In the so-called milking process of Dunaliella salina carotenoids are extracted and simultaneously produced by the culture, whilst the biomass concentration remains constant. Different theories exist about the extraction mechanisms although none have been proven yet. In this research, direct contact between dodecane and cells during the extraction process was studied microscopically and effects of direct contact were determined during in situ extraction experiments. Our results showed that water– solvent interphase contact resulted in cell death. This cell death and consequent cell rupture resulted in the release and concomitant extraction of the carotenoids. Furthermore, it has been suggested to add a small amount of dichloromethane to the biocompatible dodecane to create an organic phase with more extraction capacity. Our results showed that the addition of dichloromethane resulted in increased cell death and consequently the extraction rate increased. The improved solubility of carotenoids in an organic phase with dichloromethane did not significantly increase the extraction rate.
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