Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 403599
Title Protease digestion from wheat stillage within a dry grind ethanol facility
Author(s) Bals, B.; Brehmer, B.; Dale, B.; Sanders, J.P.M.
Source Biotechnology Progress 27 (2011)2. - ISSN 8756-7938 - p. 428 - 434.
Department(s) Biobased Chemistry and Technology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) distillers grains - extraction - proteins - optimization - hydrolysis - solubles - alcohol - options - water
Abstract As the current starch based ethanol market increases at its rapid pace, finding new markets for the primary coproduct, distiller's grains, has gained considerable interest. One possibility is to isolate the protein-rich fraction for use as precursors to biochemicals and bioplastics, further decreasing fossil fuel consumption. This research focuses on enzymatic extraction of protein peptides from wheat heavy stillage using commercially available proteases. The energy saved due to this process ranged from ~1.5 to 3.0 GJ/ton wheat stillage compared to fossil fuel-based chemicals. Using Protex 6L (Genencor), ~57% of the protein in the stillage was soluble 24 h after protease addition at 0.1% w/w loading. Of these proteins, ~32% were already soluble, indicating the importance of using wet heavy stillage as the feedstock rather than dried distiller's grains. Peptide size was less than 6 kDa. Further improvements in protein removal may be obtained through a fed batch addition of protease and improved protease cocktails
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