Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 403954
Title Acid and Base Catalyzed Hydrolysis of Cyanophycin for the Biobased Production of Nitrogen Containing Chemicals
Author(s) Könst, P.M.; Scott, E.L.; Franssen, M.C.R.; Sanders, J.P.M.
Source Journal of Biobased Materials and Bioenergy 5 (2011)1. - ISSN 1556-6560 - p. 102 - 108.
DOI http://dx.doi.org/10.1166/jbmb.2011.1126
Department(s) Biobased Chemistry and Technology
Laboratory for Organic Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) ralstonia-eutropha - saccharomyces-cerevisiae - pseudomonas-putida - amino-acids - recombinant strains - arginine - synthetase
Abstract While growing on side-streams of the agro-industries, engineered microorganisms can produce ethanol and simultaneously bind L-aspartic acid and L-arginine in equimolar amounts in the polyamino acid cyanophycin. In this way, widely available amino acids can be isolated and utilized as an alternative feedstock for the production of nitrogen containing chemicals which are nowadays based on naphtha. The first step in the envisioned route from cyanophycin towards nitrogen containing chemicals is the complete or selective hydrolysis of cyanophycin. In this study, we investigated the acid and base catalyzed hydrolysis of cyanophycin. Acid catalyzed hydrolysis of cyanophycin yields both L-aspartic acid and L-arginine at comparable rates and is therefore suitable for complete hydrolysis. Upon base catalyzed hydrolysis, the rate of L-arginine liberation is overall significantly higher than that of L-aspartic acid, which enables selective hydrolysis of cyanophycin to yield a residue with commercially interesting polyaspartic acid functionality. To conclude this study, a cyanophycin residue with reduced L-arginine content was prepared and isolated. Keywords: Cyanophycin, Hydrolysis, Amino Acids, Biobased, L-Arginine, L-Aspartic-Acid.
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