Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 404162
Title Patterning of Peptide Nucleic Acids Using Reactive Microcontact Printing
Author(s) Calabretta, A.; Wasserberg, D.; Posthuma-Trumpie, G.A.; Subramaniam, V.; Amerongen, A. van; Corradini, R.; Tedeschi, T.; Sforza, S.; Reinhoudt, D.N.; Marchelli, R.; Huskens, J.; Jonkheijm, P.
Source Langmuir 27 (2011)4. - ISSN 0743-7463 - p. 1536 - 1542.
DOI http://dx.doi.org/10.1021/la102756k
Department(s) Livestock Research
FBR Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) stereogenic centers - dna biosensors - pna - microarrays - nanoparticle - recognition - immobilization - hybridization - handedness - proteins
Abstract PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides.
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