Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 404213
Title D-2,3-Butanediol Production Due to Heterologous Expression of an Acetoin Reductase in Clostridium acetobutylicum
Author(s) Siemerink, M.A.J.; Kuit, W.; Lopez Contreras, A.M.; Eggink, G.; Oost, J. van der; Kengen, S.W.M.
Source Applied and Environmental Microbiology 77 (2011)8. - ISSN 0099-2240 - p. 2582 - 2588.
Department(s) Microbiological Laboratory
Bioprocess Engineering
FBR Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) reductase/2,3-butanediol dehydrogenase - transcriptional program - butanol fermentation - solvent production - atcc 824 - gene - biofuels - metabolism - tolerance - plasmids
Abstract Acetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions Clostridium acetobutylicum ATCC 824 (and derived strains) generates both D- and L-stereoisomers of acetoin, but due to the lack of an ACR enzyme, does not produce 2,3-butanediol. A gene encoding ACR from Clostridium beijerinckii NCIMB 8052 was functionally expressed in C. acetobutylicum under control of two strong promoters, the constitutive thl promoter and the late exponential adc promoter. Both ACR-overproducing strains were grown in batch cultures, during which 89-90% of the natively produced acetoin was converted to 20-22 mM D-2,3-butanediol. Addition of a racemic mixture of acetoin did lead to the production of both, D-2,3-butanediol and meso-2,3-butanediol. A metabolic network is proposed that is in agreement with the experimental data. Native 2,3-butanediol production is a first step towards a potential homo-fermentative 2-butanol producing strain of C. acetobutylicum.
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