Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 404310
Title Anthocyanin production as a potential visual selection marker during plant transformation
Author(s) Kortstee, A.J.; Khan, S.A.; Helderman, C.M.; Trindade, L.M.; Wu, Y.; Visser, R.G.F.; Brendolise, C.; Allan, A.C.; Schouten, H.J.; Jacobsen, E.
Source Transgenic Research 20 (2011)6. - ISSN 0962-8819 - p. 1253 - 1264.
DOI http://dx.doi.org/10.1007/s11248-011-9490-1
Department(s) Laboratory of Plant Breeding
PRI Biodiversity and Breeding
WUR Plant Breeding
EPS
EPS-3
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) transcription factor - dna transformation - transgenic plants - expression - genes - cultures - agrobacterium - biosynthesis - regeneration - efficiency
Abstract A mutant allele of the transcription factor gene MYB10 from apple induces anthocyanin production throughout the plant. This gene, including its upstream promoter, gene coding region and terminator sequence, was introduced into apple, strawberry and potato plants to determine whether it could be used as a visible selectable marker for plant transformation as an alternative to chemically selectable markers, such as kanamycin resistance. After transformation, red coloured calli, red shoots and red well-growing plants were scored. Red and green shoots were harvested from apple explants and examined for the presence of the MYB10 gene by PCR analysis. Red shoots of apple explants always contained the MYB10 gene but not all MYB10 containing shoots were red. Strawberry plants transformed with the MYB10 gene showed anthocyanin accumulation in leaves and roots. No visible accumulation of anthocyanin could be observed in potato plants grown in vitro, even the ones carrying the MYB10 gene. However, acid methanol extracts of potato shoots or roots carrying the MYB10 gene contained up to four times higher anthocyanin content than control plants. Therefore anthocyanin production as result of the apple MYB10 gene can be used as a selectable marker for apple, strawberry and potato transformation, replacing kanamycin resistance
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