Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 404937
Title Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor
Author(s) Heidmann, I.; Lange, B. de; Lambalk, J.; Angenent, G.C.; Boutilier, K.
Source Plant Cell Reports (2011). - ISSN 0721-7714 - 9 p.
DOI https://doi.org/10.1007/s00299-011-1018-x
Department(s) Laboratory of Molecular Biology
PRI BIOS Plant Development Systems
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) capsicum-annuum l. - direct somatic embryogenesis - in-vitro regeneration - plant-regeneration - agrobacterium-tumefaciens - genetic-transformation - chinense jacq. - arabidopsis - shoot - growth
Abstract Pepper (Capsicum L.) is a nutritionally and economically important crop that is cultivated throughout the world as a vegetable, condiment, and food additive. Genetic transformation using Agrobacterium tumefaciens (agrobacterium) is a powerful biotechnology tool that could be used in pepper to develop community-based functional genomics resources and to introduce important agronomic traits. However, pepper is considered to be highly recalcitrant for agrobacterium-mediated transformation, and current transformation protocols are either inefficient, cumbersome or highly genotype dependent. The main bottleneck in pepper transformation is the inability to generate cells that are competent for both regeneration and transformation. Here, we report that ectopic expression of the Brassica napus BABY BOOM AP2/ERF transcription factor overcomes this bottleneck and can be used to efficiently regenerate transgenic plants from otherwise recalcitrant sweet pepper (C. annuum) varieties. Transient activation of BABY BOOM in the progeny plants induced prolific cell regeneration and was used to produce a large number of somatic embryos that could be converted readily to seedlings. The data highlight the utility of combining biotechnology and classical plant tissue culture approaches to develop an efficient transformation and regeneration system for a highly recalcitrant vegetable crop
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.