Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 405180
Title In vitro steroidogenic effects of mixtures of persistent organic pollutants (POPs) extracted from burbot (Lota lota) caught in two Norwegian lakes
Author(s) Zimmer, K.; Montano, M.; Olsaker, I.; Dahl, E.; Berg, V.; Karlsson, C.; Murk, A.J.; Skaare, J.U.; Ropstad, E.; Verhaegen, S.
Source Science of the Total Environment 409 (2011)11. - ISSN 0048-9697 - p. 2040 - 2048.
Department(s) IMARES
Sub-department of Toxicology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) endocrine-disrupting chemicals - polybrominated diphenyl ethers - brominated flame retardants - h295r cell-line - polyhalogenated aromatic-hydrocarbons - adrenocortical carcinoma-cells - gene-expression - natural mixtures - transcriptional regulation - aldosterone
Abstract This study investigated the effects of two mixtures of persistent organic pollutants (POPs) on steroidogenesis in the H295R cell line. The two mixtures were obtained from the livers of burbot (Lota lota) caught in two Norwegian lakes (Mjøsa and Losna) with different contaminant profiles. Steroid hormone levels in the cell culture medium and mRNA levels of 16 genes involved in steroidogenesis were investigated. The crude Lake Mjøsa extract had to be diluted ten times more than the Lake Losna extract in order to prevent cytotoxicity. The ten times diluted Lake Mjøsa mixture had higher levels of DDT and derivates (¿DDTs, 1.7 times) and brominated flame retardants (¿BDEs and HBCD, 15-25 times) than the Lake Losna mixture, which, on the other hand, had higher concentrations of ¿PCBs (1.5 times higher) and also of HCB, ¿HCH isomers and ¿chlordane isomers (5-20 times higher). In the cell culture media, only cortisol levels were increased at the highest exposure concentration to the Lake Mjøsa mixture, while both cortisol and estradiol levels were increased following exposure to the two highest Lake Losna mixture exposure concentrations. Testosterone levels decreased only at the highest exposure concentration of the Lake Losna mixture. Multivariate models suggested that ¿PCBs, and to a lesser extent ¿DDTs, were responsible for the cortisol responses, while estradiol and testosterone alterations were best explained by HCB and ¿PCBs, respectively. Exposure to the mixtures generally increased mRNA levels, with smaller effects exerted by the Lake Mjøsa mixture than the Lake Losna mixture. It was concluded that both mixtures affected steroidogenesis in the H295R cells. Small differences in mixture composition, rather than the high content of brominated flame retardants in the Lake Mjøsa mixture, were suggested to be the most probable reason for the apparent differences in potencies of the two mixtures
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