Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 405639
Title Development and validation of real-time PCR screening methods for detection of cry1a.105 and cry2ab2 genes in genetically modified organisms
Author(s) Dinon, A.Z.; Prins, T.W.; Dijk, J.P. van; Arisi, C.M.; Scholtens-Toma, I.M.J.; Kok, E.J.
Source Analytical and Bioanalytical Chemistry 400 (2011)5. - ISSN 1618-2642 - p. 1433 - 1442.
Department(s) RIKILT - Business Unit Safety & Health
Rikilt B&T Novel Foods en Agroketens
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) bacillus-thuringiensis - glyphosate-tolerant - crystal proteins - reference corn - fed diets - identification - performance - gmos
Abstract Primers and probes were developed for the element-specific detection of cry1A.105 and cry2Ab2 genes, based on their DNA sequence as present in GM maize MON89034. Cry genes are present in many genetically modified (GM) plants and they are important targets for developing GMO element-specific detection methods. Element-specific methods can be of use to screen for the presence of GMOs in food and feed supply chains. Moreover, a combination of GMO elements may indicate the potential presence of unapproved GMOs (UGMs). Primer-probe combinations were evaluated in terms of specificity, efficiency and limit of detection. Except for specificity, the complete experiment was performed in 9 PCR runs, on 9 different days and by testing 8 DNA concentrations. The results showed a high specificity and efficiency for cry1A.105 and cry2Ab2 detection. The limit of detection was between 0.05 and 0.01 ng DNA per PCR reaction for both assays. These data confirm the applicability of these new primer-probe combinations for element detection that can contribute to the screening for GM and UGM crops in food and feed samples.
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