Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 405987
Title Specific in vitro toxicity of crude and refined petroleum products: 3. Estrogenic responses in mammalian assays
Author(s) Vrabie, C.M.; Candido, A.; Berg, J.H.J. van den; Murk, A.J.; Duursen, M. van; Jonker, M.T.O.
Source Environmental Toxicology and Chemistry 30 (2011)4. - ISSN 0730-7268 - p. 973 - 980.
Department(s) Sub-department of Toxicology
Aquatic Ecology and Water Quality Management
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) cancer cell-line - receptor-mediated responses - mcf-7 cells - heavy oil - er-alpha - beta - proliferation - binding - model - chemicals
Abstract Current petroleum risk assessment considers only narcosis as the mode of action, but several studies have demonstrated that oils contain compounds with dioxin-like, estrogenic or antiestrogenic, and androgenic or antiandrogenic activities. The present study is the third in a series investigating the specific toxic effects of 11 crude oils and refined products. By employing recombinant mammalian cells stably transfected with the human estrogen receptor alpha (ERa) or beta (ERß), and expressing the luciferase protein (ERa-U2OS-Luc and ERß-U2OS-Luc assay), the estrogenicity or antiestrogenicity of oils was studied. All oils, except for two refined oils and one crude oil, induced estrogenic responses. The calculated estrogenic potencies of the oils were six to nine orders of magnitude lower than the potency of 17ß-estradiol (E2). Upon coexposure to a fixed concentration of E2 and increasing concentrations of oils, additive, antagonistic, and synergistic effects were revealed. One nautical fuel oil was tested in the human breast carcinoma cell line MCF-7, in which it induced cell proliferation up to 70% relative to the maximal induction by E2. At its minimum effect concentration of 25¿mg/L, the oil was also capable of inducing mRNA expression of the estrogen-dependent protein pS2 by a factor of two. The present results indicate that oils naturally contain potentially endocrine-disrupting compounds that are able to influence the estrogenicity of other compounds and may cause biological responses beyond receptor binding.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.