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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 406041
Title Excitation energy transfer and trapping in higher plant photosystem II complexes with different antenna sizes
Author(s) Caffarri, S.; Broess, K.; Croce, R.; Amerongen, H. van
Source Biophysical Journal 100 (2011)9. - ISSN 0006-3495 - p. 2094 - 2103.
Department(s) Biophysics
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) light-harvesting complex - green plants - chlorophyll fluorescence - charge separation - arabidopsis-thaliana - core complex - kinetics - organization - membranes - lhcii
Abstract We performed picosecond fluorescence measurements on well-defined Photosystem II (PSII) supercomplexes from Arabidopsis with largely varying antenna sizes. The average excited-state lifetime ranged from 109 ps for PSII core to 158 ps for the largest C2S2M2 complex in 0.01% a-DM. Excitation energy transfer and trapping were investigated by coarse-grained modeling of the fluorescence kinetics. The results reveal a large drop in free energy upon charge separation (>700 cm-1) and a slow relaxation of the radical pair to an irreversible state (150 ps). Somewhat unexpectedly, we had to reduce the energy-transfer and charge-separation rates in complexes with decreasing size to obtain optimal fits. This strongly suggests that the antenna system is important for plant PSII integrity and functionality, which is supported by biochemical results. Furthermore, we used the coarse-grained model to investigate several aspects of PSII functioning. The excitation trapping time appears to be independent of the presence/absence of most of the individual contacts between light-harvesting complexes in PSII supercomplexes, demonstrating the robustness of the light-harvesting process. We conclude that the efficiency of the nonphotochemical quenching process is hardly dependent on the exact location of a quencher within the supercomplexes
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