Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 406058
Title Diversity arrays technology (DArT) markers in apple for genetic linkage maps
Author(s) Schouten, H.J.; Weg, W.E. van de; Carling, J.; Khan, S.A.; McKay, S.J.; Kaauwen, M.P.W. van
Source Molecular Breeding 29 (2012)3. - ISSN 1380-3743 - p. 645 - 660.
Department(s) WUR Plant Breeding
PRI Biodiversity and Breeding
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) x-domestica borkh. - quantitative trait loci - fire-blight resistance - malus-pumila mill. - venturia-inaequalis - ssr markers - assisted selection - scab resistance - wild relatives - consensus map
Abstract Diversity Arrays Technology (DArT) provides a high-throughput whole-genome genotyping platform for the detection and scoring of hundreds of polymorphic loci without any need for prior sequence information. The work presented here details the development and performance of a DArT genotyping array for apple. This is the first paper on DArT in horticultural trees. Genetic mapping of DArT markers in two mapping populations and their integration with other marker types showed that DArT is a powerful high-throughput method for obtaining accurate and reproducible marker data, despite the low cost per data point. This method appears to be suitable for aligning the genetic maps of different segregating populations. The standard complexity reduction method, based on the methylation-sensitive PstI restriction enzyme, resulted in a high frequency of markers, although there was 52-54% redundancy due to the repeated sampling of highly similar sequences. Sequencing of the marker clones showed that they are significantly enriched for low-copy, genic regions. The genome coverage using the standard method was 55-76%. For improved genome coverage, an alternative complexity reduction method was examined, which resulted in less redundancy and additional segregating markers. The DArT markers proved to be of high quality and were very suitable for genetic mapping at low cost for the apple, providing moderate genome coverage.
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