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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 406093
Title Stepwise screening of microorganisms for commercial use in biological control of plant pathogenic fungi and bacteria. Biological Control
Author(s) Köhl, J.; Postma, J.; Nicot, P.; Ruocco, M.
Source Biological Control 57 (2011)1. - ISSN 1049-9644 - p. 1 - 12.
Department(s) PRI BIOINT Ecological Interactions
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) biocontrol agents - coniothyrium-minitans - botrytis-cinerea - new-zealand - selection - antagonists - soil - rhizosphere - suppression - diseases
Abstract The development of new biocontrol products against plant diseases requires screening of high numbers of candidate antagonists. Antagonists for commercial use have to fulfill many different requirements. Besides being active against the specific targeted plant pathogens they must be safe and cost-effective. Important criteria besides pathogenic efficacy are market size, ecological characteristics, production costs, safety, environmental risks, and possibilities for intellectual property protection. A stepwise screening program considering these very different aspects is proposed. Research highlights ¿ Stepwise screening of microorganisms for commercial use in biocontrol. ¿ Multi-disciplinary approach considering many aspects besides control efficacy. ¿ Examples for protocols for use in screening programmes illustrating this approach. Article Outline 1. Introduction 2. Screening steps 2.1. Step 1: Assessment of targeted crop, disease and markets 2.2. Step 2: Origin and isolation of candidate antagonists 2.3. Step 3: Preliminary assessments in rapid-throughput screening systems 2.4. Step 4: Identification of candidate antagonists and database mining 2.5. Step 5: Efficacy testing against pathogens on plants 2.6. Step 6: Preliminary tests on mass production 2.7. Step 7: Development and testing of a pilot formulation and estimation of registration costs 2.8. Step 8: Field testing and upscaling mass production 2.9. Step 9: Integration into cropping systems Discussion
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