Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 406121
Title Engineering of Baculovirus Vectors for the Manufacture of Virion-Free Biopharmaceuticals
Author(s) Marek, M.; Oers, M.M. van; Devaraj, F.F.; Vlak, J.M.; Merten, O.W.
Source Biotechnology and Bioengineering 108 (2011)5. - ISSN 0006-3592 - p. 1056 - 1067.
Department(s) Laboratory of Virology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) nuclear polyhedrosis-virus - occlusion-derived virus - classical swine-fever - mammalian-cells - insect cells - orgyia-pseudotsugata - escherichia-coli - immune-responses - envelope protein - foreign genes
Abstract A novel baculovirus-based protein expression strategy was developed to produce recombinant proteins in insect cells without contaminating baculovirus virions. This novel strategy greatly simplifies the downstream processing of biopharmaceuticals produced in insect cells. The formation of these virions is prevented by deletion of a baculovirus gene essential for virion formation. The deletion is trans-complemented in a transgenic insect cell line in which the baculovirus seed stock is produced. The Autographa californica multicapsid nucleopolyhedrovirus vp80 gene was selected for this purpose, as absence of VP80 prevented the formation of budded virus as well as occlusion-derived virus, while foreign gene expression was not affected. Sf9 insect cells were engineered to functionally complement the vp80 deletion in the expression vector virus during seed stock production. The trans-complemented vp80-deletion baculovirus seed produced an amount of recombinant protein similar to that produced with conventional baculovirus vectors but without contaminating virions.
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