Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 406769
Title Single-tube linear DNA amplification (LinDA) for robust ChIP-seq
Author(s) Shankaranarayanan, P.; Mendoza-Parra, M.A.; Walia, M.; Wang, L.; Li, N.; Trindade, L.M.; Gronemeyer, H.
Source Nature Methods : techniques for life scientists and chemists 8 (2011). - ISSN 1548-7091 - p. 565 - 567.
DOI http://dx.doi.org/10.1038/NMETH.1626
Department(s) WUR Plant Breeding
Laboratory of Plant Breeding
EPS-3
Publication type Refereed Article in a scientific journal
Publication year 2011
Abstract Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells
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