Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 406779
Title Isolation of highly heat-resistant Listeria monocytogenes variants by use of a kinetic modeling-based sampling scheme
Author(s) Boeijen, K.H. van; Francke, C.; Moezelaar, H.R.; Abee, T.; Zwietering, M.H.
Source Applied and Environmental Microbiology 77 (2011)8. - ISSN 0099-2240 - p. 2617 - 2624.
DOI http://dx.doi.org/10.1128/AEM.02617-10
Department(s) Food Microbiology Laboratory
FBR Food Technology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) high hydrostatic-pressure - escherichia-coli - pasteurized milk - gene-expression - ctsr regulator - stress - inactivation - scott - environments - populations
Abstract Stable high-hydrostatic-pressure (HHP)-resistant Listeria monocytogenes LO28 variants were previously isolated and characterized. These HHP variants were also more resistant to heat. In addition, nonlinear heat inactivation kinetics pointed toward the existence of heat-resistant variants, although these could not be isolated so far. In this study, we used kinetic modeling of inactivation curves of two isolated HHP variants and their wild type, and this revealed that the probability of finding resistant variants should depend on the nature of the inactivation treatment and the time of exposure. At specific heat and HHP conditions, resistant LO28 and EGDe variants were indeed isolated. Resistant LO28 variants were even isolated after a heat inactivation at 72°C in milk, and these variants showed high resistance to standard pasteurization conditions. The increased resistance of part of the isolated LO28 and EGDe variants was due to mutations in their ctsR genes. For the variants whose ctsR genes and upstream regions were not altered, the mechanisms leading to increased resistance remain to be elucidated. This research showed the strength of kinetic modeling in unraveling the causes of nonlinear inactivation and facilitating the isolation of heat-resistant L. monocytogenes variants
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