Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 406799
Title Contribution of Listeria monocytogenes RecA to acid and bile survival and invasion of human intestinal Caco-2 cells
Author(s) Veen, S. van der; Abee, T.
Source International Journal of Medical Microbiology 301 (2011)4. - ISSN 1438-4221 - p. 334 - 340.
DOI http://dx.doi.org/10.1016/j.ijmm.2010.11.006
Department(s) Food Microbiology Laboratory
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) stress-response - sigma(b) contributes - salmonella-enterica - tolerance response - escherichia-coli - virulence factor - salt hydrolase - dna-damage - prfa - expression
Abstract The food-borne pathogen Listeria monocytogenes is able to colonize the human gastro-intestinal tract and subsequently cross the intestinal barrier. Thus, for L. monocytogenes to become virulent, it must survive the low pH of the stomach, high bile concentrations in the small intestine, and invade the epithelial cells. In this study, we show that RecA, which is an important factor in DNA repair and the activator of the SOS response, contributes to the resistance against acid and bile and to the ability of L. monocytogenes to adhere and invade human intestine epithelial cells. Activation of recA was shown with a promoter reporter after exposure to low pH and high bile concentrations and during adhesion and invasion of Caco-2 intestinal epithelial cells. Furthermore, an in-frame recA deletion mutant showed reduced survival after exposure to low pH and high bile concentrations. This mutant also showed a deficiency in adhesion and invasion of Caco-2 cells. These results suggest that RecA may contribute to the colonization of the human gastro-intestinal tract and crossing of the intestinal barrier. Keywords: Listeria monocytogenes; RecA; Virulence; Acid; Bile; Caco-2
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