Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 40687
Title Characterisation and modification of the exopolysaccharide produced by Lactococcus lactis subsp. cremoris B40.
Author(s) Casteren, W.H.M. van; Dijkema, C.; Schols, H.A.; Beldman, G.; Voragen, A.G.J.
Source Carbohydrate Polymers 37 (1998). - ISSN 0144-8617 - p. 123 - 130.
Department(s) Biophysics
Food Chemistry and Microbiology
Publication type Refereed Article in a scientific journal
Publication year 1998
Abstract The chemical structure of an exopolysaccharide produced by Lactococcus lactis subsp. cremoris B40 is studied, explaining earlier reported analytical discrepancies. The EPS was found to have a molecular mass of 6.8x105gmol-1 and a molar ratio of rhamnose:galactose:glucose:phosphorus of 1:1.3:2:1.1. 31P NMR indicated that a single phosphate group is present as a phosphodiester. EPS B40 was chemically modified using 0.6M H2SO4, 28M HF or 2M NaOH. From these modifications it could be concluded that galactose 1-phosphate was linked at the 3-position of 1,2,3,4-linked galactose in the backbone of the EPS. Furthermore, it appeared that during the hydrolysis step of the sugar composition analysis the galactose 3-phosphate linkages were only partially split and that, as a result, the amount of galactose was underestimated in presence of phosphate. Finally, it was demonstrated that a crude cellulase preparation was able to degrade dephosphorylated and partially de-rhamnosylated EPS.
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