Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407185
Title Structural basis for CRISPR RNA-guided DNA recognition by Cascade
Author(s) Jore, M.M.; Lundgren, N.M.J.; Duijn, E. van; Bultema, J.B.; Westra, E.R.; Oost, J. van der; Brouns, S.J.J.; Beijer, M.R.
Source Nature Structural and Molecular Biology 18 (2011)5. - ISSN 1545-9985 - p. 529 - 536.
Department(s) Microbiological Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) of-flight instrument - mass-spectrometry - r-loops - streptococcus-thermophilus - archaeoglobus-fulgidus - immune-system - protein - defense - prokaryotes - bacteria
Abstract The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids. In Escherichia coli, immunity depends on a ribonucleoprotein complex called Cascade. Here we present the composition and low-resolution structure of Cascade and show how it recognizes double-stranded DNA (dsDNA) targets in a sequence-specific manner. Cascade is a 405-kDa complex comprising five functionally essential CRISPR-associated (Cas) proteins (CasA1B2C6D1E1) and a 61-nucleotide CRISPR RNA (crRNA) with 5'-hydroxyl and 2',3'-cyclic phosphate termini. The crRNA guides Cascade to dsDNA target sequences by forming base pairs with the complementary DNA strand while displacing the noncomplementary strand to form an R-loop. Cascade recognizes target DNA without consuming ATP, which suggests that continuous invader DNA surveillance takes place without energy investment. The structure of Cascade shows an unusual seahorse shape that undergoes conformational changes when it binds target DNA
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