Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407213
Title Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins
Author(s) Dorokhin, D.; Haasnoot, W.; Franssen, M.C.R.; Zuilhof, H.; Nielen, M.W.F.
Source Analytical and Bioanalytical Chemistry 400 (2011)9. - ISSN 1618-2642 - p. 3005 - 3011.
Department(s) RIKILT - R&C Diergeneesmiddelen
Laboratory for Organic Chemistry
RIKILT - R&C Groeibevorderaars
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) linked-immunosorbent-assay - fluorescence polarization immunoassay - tandem mass-spectrometry - monoclonal-antibodies - liquid-chromatography - gas-chromatography - rapid detection - deoxynivalenol - wheat - toxin
Abstract A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin–protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 µg/kg for DON and 64 and 40 µg/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples
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