Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407264
Title Presence/absence, differential expression and sequence polymorphisms between PiAVR2 and PiAVR2-like in Phytophthora infestans determine virulence on R2 plants
Author(s) Gilroy, E.M.; Breen, S.; Whisson, S.C.; Squires, J.; Hein, I.; Kaczmarek, M.; Turnbull, D.; Boevink, P.C.; Lokossou, A.A.; Cano, L.M.; Morales, J.; Avrova, A.O.; Pritchard, L.; Randall, E.; Lees, A.; Govers, F.; West, P. van; Kamoun, S.; Vleeshouwers, V.G.A.A.; Cooke, D.E.L.; Birch, P.R.J.
Source New Phytologist 191 (2011)3. - ISSN 0028-646X - p. 763 - 776.
Department(s) Laboratory of Plant Breeding
Laboratory of Phytopathology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) rxlr effectors - pathogen phytophthora - disease resistance - oomycete effectors - avirulence genes - downy mildew - potato - locus - avr3a - protein
Abstract # A detailed molecular understanding of how oomycete plant pathogens evade disease resistance is essential to inform the deployment of durable resistance (R) genes. # • Map-based cloning, transient expression in planta, pathogen transformation and DNA sequence variation across diverse isolates were used to identify and characterize PiAVR2 from potato late blight pathogen Phytophthora infestans. # • PiAVR2 is an RXLR-EER effector that is up-regulated during infection, accumulates at the site of haustoria formation, and is recognized inside host cells by potato protein R2. Expression of PiAVR2 in a virulent P. infestans isolate conveys a gain-of-avirulence phenotype, indicating that this is a dominant gene triggering R2-dependent disease resistance. PiAVR2 presence/absence polymorphisms and differential transcription explain virulence on R2 plants. Isolates infecting R2 plants express PiAVR2-like, which evades recognition by R2. PiAVR2 and PiAVR2-like differ in 13 amino acids, eight of which are in the C-terminal effector domain; one or more of these determines recognition by R2. Nevertheless, few polymorphisms were observed within each gene in pathogen isolates, suggesting limited selection pressure for change within PiAVR2 and PiAVR2-like. # • Our results direct a search for R genes recognizing PiAVR2-like, which, deployed with R2, may exert strong selection pressure against the P. infestans population.
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