Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407316
Title Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs
Author(s) Brookes, S.M.; Nunez, A.; Choudhury, B.; Matrosovich, M.; Essen, S.C.; Clifford, D.; Slomka, M.J.; Kuntz-Simon, G.; Garcon, F.; Nash, B.; Hanna, A.; Heegaard, P.M.H.; Queguiner, S.; Chiapponi, C.; Bublot, M.; Garcia, J.M.; Gardner, R.; Foni, E.; Loeffen, W.L.A.; Larsen, L.; Reeth, K.; Banks, J.; Irvine, R.M.; Brown, I.H.
Source PLoS One 5 (2010)2. - ISSN 1932-6203 - 9 p.
Department(s) CVI Virology
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) avian influenza-viruses - acute-phase protein - receptor-binding properties - c-reactive protein - interspecies transmission - experimental-infection - mdck cells - hemagglutinin - strains - ferrets
Abstract The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions, including origin and host range [1,2]. Two of the three pandemics in the last century resulted in the spread of virus to pigs (H1N1, 1918; H3N2, 1968) with subsequent independent establishment and evolution within swine worldwide [3]. A key public and veterinary health consideration in the context of the evolving pandemic is whether the H1N1/09 virus could become established in pig populations [4]. We performed an infection and transmission study in pigs with A/California/07/09. In combination, clinical, pathological, modified influenza A matrix gene real time RT-PCR and viral genomic analyses have shown that infection results in the induction of clinical signs, viral pathogenesis restricted to the respiratory tract, infection dynamics consistent with endemic strains of influenza A in pigs, virus transmissibility between pigs and virus-host adaptation events. Our results demonstrate that extant H1N1/09 is fully capable of becoming established in global pig populations. We also show the roles of viral receptor specificity in both transmission and tissue tropism. Remarkably, following direct inoculation of pigs with virus quasispecies differing by amino acid substitutions in the haemagglutinin receptor-binding site, only virus with aspartic acid at position 225 (225D) was detected in nasal secretions of contact infected pigs. In contrast, in lower respiratory tract samples from directly inoculated pigs, with clearly demonstrable pulmonary pathology, there was apparent selection of a virus variant with glycine (225G). These findings provide potential clues to the existence and biological significance of viral receptor-binding variants with 225D and 225G during the 1918 pandemic [5].
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