Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 407318
Title Grafted ionomer complexes and their effect on protein adsorption on silica and polysulfone surfaces
Author(s) Brzozowska, A.M.; Keizer, A. de; Detrembleur, C.; Stuart, M.A.C.; Norde, W.
Source Colloid and Polymer Science 288 (2010)16-17. - ISSN 0303-402X - p. 1621 - 1632.
Department(s) Physical Chemistry and Colloid Science
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) coacervate core micelles - pyridinium-type polymers - bovine serum-albumin - polyelectrolyte multilayers - polyvinyl pyridine - ultrafiltration membranes - antibacterial activity - polystyrene surfaces - aqueous-solutions - block-copolymer
Abstract We have studied the formation and the stability of ionomer complexes from grafted copolymers (GICs) in solution and the influence of GIC coatings on the adsorption of the proteins beta-lactoglobulin (beta-lac), bovine serum albumin (BSA), and lysozyme (Lsz) on silica and polysulfone. The GICs consist of the grafted copolymer PAA(28)-co-PAPEO(22) {poly(acrylic acid)-co-poly[acrylate methoxy poly(ethylene oxide)]} with negatively charged AA and neutral APEO groups, and the positively charged homopolymers: P2MVPI(43) [poly(N-methyl 2-vinyl pyridinium iodide)] and PAH center dot HCl160 [poly(allylamine hydrochloride)]. In solution, these aggregates are characterized by means of dynamic and static light scattering. They appear to be assemblies with hydrodynamic radii of 8 nm (GIC-PAPEO(22)/P2MVPI(43)) and 22 nm (GIC-PAPEO(22)/PAH center dot HCl160), respectively. The GICs partly disintegrate in solution at salt concentrations above 10 mM NaCl. Adsorption of GICs and proteins has been studied with fixed angle optical reflectometry at salt concentrations ranging from 1 to 50 mM NaCl. Adsorption of GICs results in high density PEO side chains on the surface. Higher densities were obtained for GICs consisting of PAH center dot HCl160 (1.6 divided by 1.9 chains/nm(2)) than of P2MVPI(43) (0.6 divided by 1.5 chains/nm(2)). Both GIC coatings strongly suppress adsorption of all proteins on silica (>90%); however, reduction of protein adsorption on polysulfone depends on the composition of the coating and the type of protein. We observed a moderate reduction of beta-lac and Lsz adsorption (>60%). Adsorption of BSA on the GIC-PAPEO(22)/ P2MVPI(43) coating is moderately reduced, but on the GIC-PAPEO(22)/ PAH center dot HCl160 coating it is enhanced.
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