Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
Record number 407339
Title Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols
Author(s) Abzac, P. D'; Bordas, F.; Hullebusch, E.; Lens, P.N.L.; Guibaud, G.
Source Applied Microbiology and Biotechnology 85 (2010)5. - ISSN 0175-7598 - p. 1589 - 1599.
DOI http://dx.doi.org/10.1007/s00253-009-2288-x
Department(s) Sub-department of Environmental Technology
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) activated-sludge - waste-water - polysaccharides - complexation - flocs - acid
Abstract The characteristics of the extracellular polymeric substances (EPS) extracted with nine different extraction protocols from four different types of anaerobic granular sludge were studied. The efficiency of four physical (sonication, heating, cationic exchange resin (CER), and CER associated with sonication) and four chemical (ethylenediaminetetraacetic acid, ethanol, formaldehyde combined with heating, or NaOH) EPS extraction methods was compared to a control extraction protocols (i.e., centrifugation). The nucleic acid content and the protein/polysaccharide ratio of the EPS extracted show that the extraction does not induce abnormal cellular lysis. Chemical extraction protocols give the highest EPS extraction yields (calculated by the mass ratio between sludges and EPS dry weight (DW)). Infrared analyses as well as an extraction yield over 100% or organic carbon content over 1 g g(-1) of DW revealed, nevertheless, a carry-over of the chemical extractants into the EPS extracts. The EPS of the anaerobic granular sludges investigated are predominantly composed of humic-like substances, proteins, and polysaccharides. The EPS content in each biochemical compound varies depending on the sludge type and extraction technique used. Some extraction techniques lead to a slightly preferential extraction of some EPS compounds, e.g., CER gives a higher protein yield.
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.