Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 407346
Title Determination of fumonisins B-1 and B-2 in maize-based baby food products by HPLC with fluorimetric detection after immunoaffinity column clean-up
Author(s) Girolamo, A. De; Pereboom-de Fauw, D.P.K.H.; Sizoo, E.; Egmond, H.P. van; Gambacorta, L.; Bouten, K.; Stroka, J.; Visconti, A.; Solfrizzo, M.
Source World Mycotoxin Journal 3 (2010)2. - ISSN 1875-0710 - p. 135 - 146.
DOI http://dx.doi.org/10.3920/WMJ2010.1213
Department(s) RIKILT - R&C Natuurlijke Toxinen en Pesticiden
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) chromatography/tandem mass-spectrometry - corn-based foods - liquid-chromatography - extraction - mycotoxins - cornflakes - stability
Abstract A method for the determination of fumonisin B-1 (FB1) and B-2 (FB2) in different commercial maize-based products for infants and young children was developed and tested in a limited validation study involving 3 laboratories. The method used extraction at 55 C with an acidic mixture of methanol-acetonitrile-phosphate/citrate buffer, clean-up through immunoaffinity column and fumonisin determination by high performance liquid chromatography with automated pre-column derivatisation with 0-phthaldialdehyde. Recovery experiments were performed at five spiking levels in the ranges of 80-800 mu g/kg FB1 and 20-200 mu g/kg FB2. Mean recoveries ranged from 83 to 97% for FBI and from 61 to 78% for FB2. Relative standard deviations for within-laboratory repeatability (RSDr.) ranged from 5 to 12% for FBI and from 8 to 13% for FB2, whereas relative standard deviation for between-laboratory reproducibility (RSDR) ranged from 6 to 10% for FBI and from 9 to 16% for FB2. The limit of quantification of the method (signal to noise ratio of 6) was 2.8 mu g/kg for FBI and 2.2 mu g/kg for FB2. Fumonisins were found in 6 out of 19 maize-based baby foods obtained from the Italian retail market at levels up to 53 mu g/kg.
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