Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407350
Title DNA condensation in bacteria: Interplay between macromolecular crowding and nucleoid proteins
Author(s) Vries, R.J. de
Source Biochimie 92 (2010)12. - ISSN 0300-9084 - p. 1715 - 1721.
DOI https://doi.org/10.1016/j.biochi.2010.06.024
Department(s) Physical Chemistry and Colloid Science
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) escherichia-coli - bundle formation - f-actin - compaction - cytoplasm - chromatin - filaments - archaea - complex - systems
Abstract The volume of a typical Eschericia coli nucleoid is roughly 10(4) times smaller than the volume of a freely coiling linear DNA molecule with the same length as the E. coli genome. We review the main forces that have been suggested to contribute to this compaction factor: macromolecular crowding (that "pushes" the DNA together), DNA charge neutralization by various polycationic species (that "glues" the DNA together), and finally, DNA deformations due to DNA supercoiling and nucleoid proteins. The direct contributions of DNA supercoiling and nucleoid proteins to the total compaction factor are probably small. Instead, we argue that the formation of the bacterial nucleoid can be described as a consequence of the influence of macromolecular crowding on thick, supercoiled protein-DNA fibers, that have been partly charge neutralized by small multivalent cations.
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