Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407365
Title Potato R1 resistance gene confers resistance against Phytophthora infestans in transgenic tomato plants
Author(s) Faino, L.; Carli, P.; Testa, A.; Cristinzio, G.; Frusciante, L.; Ercolano, M.R.
Source European Journal of Plant Pathology 128 (2010)2. - ISSN 0929-1873 - p. 233 - 241.
Department(s) WUR Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) late blight resistance - real-time pcr - copy number - dna fragments - mosaic-virus - transformation - zygosity - tobacco - expression - efficient
Abstract Tomato is challenged by several pathogens which cause loss of production. One such pathogen is the oomycete Phytophthora infestans which is able to attack all the aerial parts of the plant. Although a wide range of resistance sources are available, genetic control of this disease is not yet successful. Pyramiding R-genes through genetic transformation could be a straightforward way to produce tomato and potato lines carrying durable resistance to P. infestans. In this work the R1 potato gene was transferred into tomato lines. The tomato transgenic lines were analyzed by using q-RT-PCR and progeny segregation to determine the gene copy number. To test the hypothesis that R1 represents a specifically regulated R-gene, transgenic tomato plants were inoculated with P. infestans isolate 88133 and IPO. All the plants containing the R1 gene were resistant to the late blight isolate IPO-0 and susceptible to isolate 88133. These results provide evidence for specific activation of the R1 gene during pathogen challenge. Furthermore, evidence for enhancement of PR-1 gene expression during P. infestans resistance response was obtained.
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