Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 407410
Title Ultraslow microdialysis and microfiltration for in-line, on-line and off-line monitoring
Author(s) Korf, J.; Huinink, K.D.; Posthuma-Trumpie, G.A.
Source Trends in Biotechnology 28 (2010)3. - ISSN 0167-7799 - p. 150 - 158.
Department(s) Livestock Research
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) mass-spectrometric proteomics - ultrafiltration probes - quantitative microdialysis - protein-adsorption - inflammatory response - blood compatibility - vivo microdialysis - adipose-tissue - hollow-fiber - lactate
Abstract In medicine and biotechnology, close monitoring of molecular processes might assist to optimise therapeutic interventions and production of biochemicals, respectively. Here, we summarize the current status of two automatic and continuous sampling technologies, microdialysis and microfiltration, which facilitate both in vivo and in vitro monitoring of nearly any analyte, because they can be combined easily with many analytical techniques. Conventional microdialysis and microfiltration, which require collecting relatively large samples, are however often impractical and semi-quantitative; hence, we focus on ultraslow sampling to circumvent such limitations. Ultraslow microdialysis and microfiltration already have been used successfully for quantitative pharmacokinetics, glucose metabolism (e.g. of the brain), cytokines and proteomics (e.g. tumour secretomes), both in vivo and in vitro.
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