Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407425
Title Antibiotic susceptibility of members of the Lactobacillus acidophilus group using broth microdilution and molecular identification of their resistance determinants
Author(s) Mayrhofer, S.; Hoek, A.H.A.M. van; Mair, C.; Huys, G.; Aarts, H.J.M.; Kneifel, W.; Domig, K.J.
Source International Journal of Food Microbiology 144 (2010)1. - ISSN 0168-1605 - p. 81 - 87.
DOI http://dx.doi.org/10.1016/j.ijfoodmicro.2010.08.024
Department(s) RIKILT - V&G Microbiologie & Novel Foods
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) lactic-acid bacteria - antimicrobial susceptibility - safety - genes - food - bifidobacteria - probiotics - strains - origin - pediococcus
Abstract The range of antibiotic susceptibility to 13 antibiotics in 101 strains of the Lactobacillus acidophilus group was examined using the lactic acid bacteria susceptibility test medium (LSM) and broth microdilution. Additionally, microarray analysis and PCR were applied to identify resistance genes responsible for the displayed resistant phenotypes in a selection of strains. In general, narrow as well as broad unimodal and bimodal MIC distributions were observed for the Lactobacillus acidophilus group and the tested antimicrobial agents. Atypically resistant strains could be determined by visual inspection of the obtained MIC ranges for ampicillin, chloramphenicol, clindamycin, erythromycin, quinupristin/dalfopristin, streptomycin and tetracycline. For most of these atypically resistant strains underlying resistance determinants were found. To our knowledge erm(A) was detected in lactobacilli for the first time within this study. Data derived from this study can be used as a basis for reviewing present microbiological breakpoints for categorization of susceptible and resistant strains within the Lactobacillus acidophilus group to assess the safety of microorganisms intended for use in food and feed applications
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