Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407434
Title Child hospitalization due to severe malaria is associated with the ICAM-1(Kilifi) allele but not adherence patterns of Plasmodium falciparum infected red blood cells to ICAM-1
Author(s) Mwanziva, C.; Mpina, M.; Balthazary, S.; Mkali, H.; Mbugi, E.V.; Mosha, F.; Chilongola, J.
Source Acta Tropica 116 (2010)1. - ISSN 0001-706X - p. 45 - 50.
Department(s) Cell Biology and Immunology
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) intercellular-adhesion molecule-1 - rhinovirus receptor - clinical severity - cerebral malaria - binding-sites - polymorphism - protein - domain - sequestration - cytoadherence
Abstract This study aimed at determining whether the predisposition of a mutation at position 179 of the ICAM-1 gene to child hospitalization due to malaria was mediated by changes in adherence properties of IRBCs to ICAM-1. ICAM-1 genotypes were determined by nested polymerase chain reaction of isolated DNA from filter blood spots followed by Restriction Fragment Length Polymorphism (RFLP). Plasmodium falciparum adherence assays were done on immobilized purified ICAM-1. Our data indicate that the homozygosity for the ICAM-1(Kilifi) mutation occurs at a frequency of 22.3% in Magugu-Babati, Northern Tanzania. Our results show that there are no differences in IRBC binding profiles across genotypes. We show in this study that homozygosity for the ICAM-1(Kilifi) is associated with child hospitalization (X-2 = 14.47, p <0.001). We have further shown that hospitalization was not associated with cytoadherence (X-2 = 0.17, p = 0.68). We conclude that the ICAM-1(Kilifi) allele occurs at a high frequency in Tanzania and that associations of this allele with higher child hospitalization frequencies is independent of cytoadherence patterns of IRBC isolated from ICAM-1 genotypes, implying that any associations reported to exist between the ICAM-1(Kilifi) mutation and severe malaria are unlikely to be mediated through altered IRBC cytoadherence properties.
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