Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 407524
Title An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR
Author(s) Star, W.R.L. van der; Dijkema, C.; Waard, P. de; Picioreanu, C.; Strous, M.; Loosdrecht, M.C.M. van
Source Applied Microbiology and Biotechnology 86 (2010)1. - ISSN 0175-7598 - p. 311 - 317.
Department(s) Biophysics
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) ammonium-oxidizing bacteria - nuclear-magnetic-resonance - proton gradients - 4 genera - compartmentation - enrichment - oxidation - reactor - cells - identification
Abstract The cytoplasm of anaerobic ammonium oxidizing (anammox) bacteria consists of three compartments separated by membranes. It has been suggested that a proton motive force may be generated over the membrane of the innermost compartment, the "anammoxosome". P-31 nuclear magnetic resonance (NMR) spectroscopy was employed to investigate intracellular pH differences in the anammox bacterium Kuenenia stuttgartiensis. With in vivo NMR, spectra were recorded of active, highly concentrated suspensions of K. stuttgartiensis in a wide-bore NMR tube. At different external pH values, two stable and distinct phosphate peaks were apparent in the recorded spectra. These peaks were equivalent with pH values of 7.3 and 6.3 and suggested the presence of a proton motive force over an intracytoplasmic membrane in K. stuttgartiensis. This study provides for the second time-after discovery of acidocalcisome-like compartments in Agrobacterium tumefaciens-evidence for an intracytoplasmic pH gradient in a chemotrophic prokaryotic cell
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