Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 407915
Title Different Mycobacterium avium subsp. paratuberculosis MIRU-VNTR patterns coexist within cattle herds
Author(s) Hulzen, K.J.E. van; Heuven, H.C.M.; Nielen, M.; Hoeboer, J.; Santema, W.J.; Koets, A.P.
Source Veterinary Microbiology 148 (2011)2-4. - ISSN 0378-1135 - p. 419 - 424.
DOI http://dx.doi.org/10.1016/j.vetmic.2010.09.029
Department(s) Animal Breeding and Genetics
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) number tandem-repeat - genetic diversity - genome - tuberculosis - population - evolution - strains - complex - element - is1245
Abstract A better understanding of the biodiversity of Mycobacterium avium subsp. paratuberculosis (MAP) offers more insight in the epidemiology of paratuberculosis and therefore may contribute to the control of the disease. The aim of this study was to investigate the genetic diversity in bovine MAP isolates using PCR-based methods detecting genetic elements called Variable-Number Tandem Repeats (VNTRs) and Mycobacterial Interspersed Repetitive Units (MIRUs) to determine if multiple MAP strains can coexist on farms with endemic MAP infection. For 52 temporal isolates originating from infected cattle from 32 commercial dairy herds with known trading history, MIRU–VNTR analysis was applied at 10 loci of which six showed variation. Within the group of 52 isolates, 17 different MIRU–VNTR patterns were detected. One MIRU–VNTR pattern was found in 29 isolates, one pattern in four isolates, one pattern in three isolates, two times one MIRU–VNTR pattern was found occurring in two isolates, and 12 patterns were found only once. Eleven herds provided multiple isolates. In five herds a single MIRU–VNTR pattern was detected among multiple isolates whereas in six herds more than one pattern was found. This study confirms that between dairy farms as well as within dairy farms, infected animals shed MAP with different MIRU–VNTR patterns. Analysis of trading history and age within herds indicated that cows born within the same birth cohort can be infected with MAP strains exhibiting variations in the number of MIRU–VNTR repeats. These data indicate that such multiple genotypes of MAP can coexist within one herd.
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.