Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 408179
Title Gene transcription analysis during interaction between potato and Ralstonia solanacearum
Author(s) Li, G.C.; Jin, L.P.; Wang, X.W.; Xie, K.Y.; Yang, Y.; Vossen, E.A.G. van der; Huang, S.W.; Qu, D.Y.
Source Russian Journal of Plant Physiology 57 (2010)5. - ISSN 1021-4437 - p. 685 - 695.
Department(s) PRI Biodiversity and Breeding
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) hypersensitive response - bacterial wilt - chitinase gene - resistance - arabidopsis - domain - expression - kinase - rice - phosphatase
Abstract Bacterial wilt (BW) caused by Ralstonia solanacearum (Rs) is an important quarantine disease that spreads worldwide and infects hundreds of plant species. The BW defense response of potato is a complicated continuous process, which involves transcription of a battery of genes. The molecular mechanisms of potato-Rs interactions are poorly understood. In this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differentially expressed during the incompatible interaction between Rs and potato. In total, 302 differentially expressed genes were identified and classified into 12 groups according to their putative biological functions. Of 302 genes, 81 were considered as Rs resistance-related genes based on the homology to genes of known function, and they have putative roles in pathogen recognition, signal transduction, transcription factor functioning, hypersensitive response, systemic acquired resistance, and cell rescue and protection. Additionally, 50 out of 302 genes had no match or low similarity in the NCBI databases, and they may represent novel genes. Of seven interesting genes analyzed via RNA gel blot and semi-quantitative RT-PCR, six were induced, one was suppressed, and all had different transcription patterns. The results demonstrate that the response of potato against Rs is rapid and involves the induction of numerous various genes. The genes identified in this study add to our knowledge of potato resistance to Rs.
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