Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 408197
Title Dietary calcium decreases but short-chain fructo-oligosaccharides increase colonic permeability in rats
Author(s) Schepens, M.A.A.; Rijnierse, A.; Schonewille, A.J.; Vink, C.; Brummer, R.J.M.; Willemsen, L.E.M.; Meer, R. van de; Bovee-Oudenhoven, I.M.J.
Source British Journal of Nutrition 104 (2010)12. - ISSN 0007-1145 - p. 1780 - 1786.
DOI http://dx.doi.org/10.1017/S0007114510002990
Department(s) Chair Nutrition Metabolism and Genomics
RIKILT - Business Unit Safety & Health
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) intestinal permeability - ulcerative-colitis - crohns-disease - fatty-acids - bile-acids - inulin - salmonella - humans - oligofructose - translocation
Abstract An increased intestinal permeability is associated with several diseases. Nutrition can influence gut permeability. Previously, we showed that dietary Ca decreases whereas dietary short-chain fructo-oligosaccharides (scFOS) increase intestinal permeability in rats. However, it is unknown how and where in the gastrointestinal tract Ca and scFOS exert their effects. Rats were fed a Western low-Ca control diet, or a similar diet supplemented with either Ca or scFOS. Lactulose plus mannitol and Cr-EDTA were added to the diets to quantify small and total gastrointestinal permeability, respectively. Additionally, colonic tissue was mounted in Ussing chambers and exposed to faecal water of these rats. Dietary Ca immediately decreased urinary Cr-EDTA excretion by 24 % in Ca-fed rats compared with control rats. Dietary scFOS increased total Cr-EDTA permeability gradually with time, likely reflecting relatively slow gut microbiota adaptations, which finally resulted in a 30 % increase. The lactulose: mannitol ratio was 15 % higher for Ca-fed rats and 16 % lower for scFOS-fed rats compared with control rats. However, no dietary effect was present on individual urinary lactulose and mannitol excretion. The faecal waters did not influence colonic permeability in Ussing chambers. In conclusion, despite effects on the lactulose: mannitol ratio, individual lactulose values did not alter, indicating that diet did not influence small-intestinal permeability. Therefore, both nutrients affect permeability only in the colon: Ca decreases, while scFOS increase colonic permeability. As faecal water did not influence permeability in Ussing chambers, probably modulation of mucins and/or microbiota is important for the in vivo effects of dietary Ca and scFOS.
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