Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 408206
Title Optimization and prevalidation of the in vitro ER alpha CALUX method to test estrogenic and antiestrogenic activity of compounds
Author(s) Burg, B. van der; Winter, R.; Weimer, M.; Berckmans, P.; Suzuki, G.; Gijsbers, L.; Jonas, A.; Linden, S. van der; Witters, H.; Aarts, J.M.M.J.G.; Legler, J.; Kopp-Schneider, A.; Bremer, S.
Source Reproductive Toxicology 30 (2010)1. - ISSN 0890-6238 - p. 73 - 80.
Department(s) Sub-department of Toxicology
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) cell-line - transactivation assay - endocrine disruptors - bioassays - androgen - chemicals - panel - beta
Abstract Estrogenicity of chemicals has received significant attention and is linked to endocrine-disrupting activities. However, there is a paucity of validated methods to assess estrogenicity in vitro. We have established a robust method to test estrogenic and antiestrogenic activity of compounds in vitro, as an alternative to using animal models such as the uterotrophic assay. To this end we optimized protocols to be used in combination with CALUX reporter gene assays and carried out an in house prevalidation, followed by two rounds of tests to establish transferability. Problems in the initial test with transferability were solved by isolation of a novel cell clone of the ER alpha CALUX line with greatly improved stability and luciferase levels. This cell line proved to be a very suitable and reliable predictor of estrogenicity of chemicals and was able to readily rank a range of chemicals on the basis of their EC50 values.
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