Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 408437
Title Biosynthesis and localization of parthenolide in glandular trichomes of feverfew (Tanacetum parthenium L. Schulz Bip.)
Author(s) Majdi, M.; Liu, Q.; Karimzadeh, G.; Malboobi, M.A.; Beekwilder, J.; Cankar, K.; Vos, C.H. de; Todorovic, S.; Simonovic, A.; Bouwmeester, H.J.
Source Phytochemistry 72 (2011)14-15. - ISSN 0031-9422 - p. 1739 - 1750.
Department(s) Laboratory of Plant Physiology
PRI BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) germacrene-a synthase - artemisia-annua l - sesquiterpene lactones - gene-expression - amorpha-4,11-diene synthase - developmental regulation - migraine prophylaxis - double-blind - in-vitro - asteraceae
Abstract Feverfew (Tanacetum parthenium) is a perennial medicinal herb and is a rich source of sesquiterpene lactones. Parthenolide is the main sesquiterpene lactone in feverfew and has attracted attention because of its medicinal potential for treatment of migraine and cancer. In the present work the parthenolide content in different tissues and developmental stages of feverfew was analyzed to study the timing and localization of parthenolide biosynthesis. The strongest accumulating tissue was subsequently used to isolate sesquiterpene synthases with the goal to isolate the gene encoding the first dedicated step in parthenolide biosynthesis. This led to the isolation and charachterization of a germacrene A synthase (TpGAS) and an (E)-ß-caryophyllene synthase (TpCarS). Transcript level patterns of both sesquiterpene synthases were analyzed in different tissues and glandular trichomes. Although TpGAS was expressed in all aerial tissues, the highest expression was observed in tissues that contain high concentrations of parthenolide and in flowers the highest expression was observed in the biosynthetically most active stages of flower development. The high expression of TpGAS in glandular trichomes which also contain the highest concentration of parthenolide, suggests that glandular trichomes are the secretory tissues where parthenolide biosynthesis and accumulation occur.
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