Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 408516
Title Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus
Author(s) Kozarski, M.; Klaus, A.; Niksic, M.; Jakovljevic, D.; Helsper, J.P.F.G.; Griensven, L.J.L.D. van
Source Food Chemistry 129 (2011)4. - ISSN 0308-8146 - p. 1667 - 1675.
DOI http://dx.doi.org/10.1016/j.foodchem.2011.06.029
Department(s) PRI BIOS Applied Genomics & Proteomics
PRI BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) edible mushrooms - interferon-gamma - fruiting bodies - tyrosinase - antitumor - monophenols - phenols - glucans - water
Abstract Partially purified polysaccharides were obtained from four medicinal mushroom species, Agaricus bisporus, Agaricus brasiliensis, Phellinus linteus and Ganoderma lucidum by hot water extraction, followed by ethanol precipitation. The four samples contained varying amounts of both a- and ß-glucans as determined by FT-IR and by quantitative estimation after prior partial hydrolysis (Megazyme ß-glucan assay kit). EC50 values of the DPPHradical dot scavenging activity of the polysaccharides from G. lucidum spores and P. linteus fruiting bodies were found to be particularly low, i.e. EC50 <0.1 mg/ml. For A. brasiliensis and A. bisporus, EC50 values were 0.27 and 2.0 mg/ml. EC50 values of the antioxidant activity were 7.07 mg/ml for G. lucidum, 13.25 mg/ml for A. brasiliensis and >20 mg/ml for A. bisporus polysaccharide, respectively. EC50 values of the chelating activity of ferrous ions ranged from 0.59 mg/ml for G. lucidum to 7.80 mg/ml for A. bisporus. The EC50 values of the extracts in the reducing power assay ranged from 0.47 to 14.83 mg/ml. A correlation was found between EC50 values of the chelating and reducing power abilities and the amount of total glucans content in the extracts. In vitro measurements of immunomodulatory capacity of polysaccharide extracts showed that A. bisporus, A. brasiliensis fruiting bodies and G. lucidum spores extracts express an immunostimulating effect on activated human PBMCs and induce synthesis of IFN-¿. The polysaccharide extract of P. linteus fruiting bodies showed an immunosuppressive effect.
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