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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 408517
Title Yeast Protein–Protein Interaction Assays and Screens
Author(s) Folter, S. de; Immink, G.H.
Source In: Plant Transcription Factors, Methods in Molecular Biology / Yuan, L., Perry, S.E., Springer Science (Methods in Molecular Biology 754) - p. 145 - 165.
DOI https://doi.org/10.1007/978-1-61779-154-3_8
Department(s) PRI Bioscience
PRI BIOS Plant Development Systems
Publication type Peer reviewed book chapter
Publication year 2011
Abstract Most transcription factors fulfill their role in protein complexes. As a consequence, information about their interaction capacity sheds light on a protein’s function and the molecular mechanism underlying this activity. The yeast two-hybrid GAL4 (Y2H) assay is a powerful method to unravel and identify the composition of protein complexes. This in vivo based system makes use of two functional protein domains of the GAL4 transcription factor, each fused to a protein of interest. Upon interaction between the two proteins under study, a transcriptional activator gets reconstituted and reporter genes get activated, allowing the yeast to grow on selective medium. In this chapter protocols are given for Y2H library screening, directed Y2H screening, Y2H matrix screening, and YnH screening involving more than two proteins.
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