Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 408518
Title Preferential use of RNA leader sequences during influenza A transcription initiation in vivo
Author(s) Geerts-Dimitriadou, C.; Goldbach, R.W.; Kormelink, R.J.M.
Source Virology 409 (2011)1. - ISSN 0042-6822 - p. 27 - 32.
DOI http://dx.doi.org/10.1016/j.virol.2010.09.006
Department(s) Laboratory of Virology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) b-virus genome - complete nucleotide-sequence - cap-snatching mechanism - open reading frame - messenger-rna - viral-rna - 5' ends - base complementarity - neuraminidase gene - alpha-amanitin
Abstract In vitro transcription initiation studies revealed a preference of influenza A virus for capped RNA leader sequences with base complementarity to the viral RNA template. Here, these results were verified during an influenza infection in MDCK cells. Alfalfa mosaic virus RNA3 leader sequences mutated in their base complementarity to the viral template, or the nucleotides 5' of potential base-pairing residues, were tested for their use either singly or in competition. These analyses revealed that influenza transcriptase is able to use leaders from an exogenous mRNA source with a preference for leaders harboring base complementarity to the 3'-ultimate residues of the viral template, as previously observed during in vitro studies. Internal priming at the 3'-penultimate residue, as well as “prime-and-realign” was observed. The finding that multiple base-pairing promotes cap donor selection in vivo, and the earlier observed competitiveness of such molecules in vitro, offers new possibilities for antiviral drug design.
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