Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 408565
Title Do plastids in Dendrobium cv. Lucky Duan petals function similar to autophagosomes and autolysosomes
Author(s) Doorn, W.J. van; Kirasak, K.; Sonong, A.; Srihiran, Y.; Lent, J.W.M. van; Ketsa, S.
Source Autophagy 7 (2011)6. - ISSN 1554-8627 - p. 584 - 597.
DOI http://dx.doi.org/10.4161/auto.7.6.15099
Department(s) Laboratory of Virology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) acid-phosphatase-activity - cell-death - arabidopsis-thaliana - embryo-suspensor - protein - stromules - membrane - vacuoles - plants - visualization
Abstract In animal cells a double-membrane-bound structure, the autophagosome, encloses a portion of the cytoplasm. The encapsulated material becomes digested after fusion of the autophagosome with a vesicle containing lytic enzymes. The autophagosome is then termed autolysosome. In intact plants, structures similar to animal autophagosomes/autolysosomes have been found only in a few types of cells. Additionally, some early papers indicated that plastids can function similar to autophagosomes/autolysosomes. Here, we report that plastids in Dendrobium cv. Lucky Duan petals produced an endocytosis-like invagination of the two outer membranes. The opening between the invagination space and the cytoplasm was almost isodiametric, less than 0.2 µm in diameter. The volume of the space formed by the invagination had a maximum of about half of the total plastid volume. Staining of the invagination lumen for acid phosphatase, a marker of organelles showing autophagic activity, was positive. Membranes and numerous ribosomes were observed inside the lumen of the invagination. The structure of the material inside the lumen varied from that of the cytoplasm to uniform electron-translucent, indicating that the enclosed cytoplasmic material became completely digested. No support was found for the idea that the material engulfed by the plastid or the whole plastid became transferred to a vacuole. Taken together, the data suggested the hypothesis that plastids in Dendrobium petal mesophyll cells can function in a way similar to both autophagosomes and autolysosomes in animal cells
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