Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 409067
Title Recombinant cell bioassays for the detection of (gluco) corticosteroids and endocrine-disrupting potencies of several enviromental PCB contaminants
Author(s) Bovee, T.F.H.; Helsdingen, J.R.; Hamers, A.R.M.; Brouwer, B.A.; Nielen, M.W.F.
Source Analytical and Bioanalytical Chemistry 401 (2011)3. - ISSN 1618-2642 - p. 873 - 882.
Department(s) RIKILT - Business Unit Safety & Health
Laboratory for Organic Chemistry
RIKILT - R&C Groeibevorderaars
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) human glucocorticoid-receptor - green fluorescent protein - in-vitro - androgen receptor - growth promoters - beta-isoform - progesterone - expression - gene - transcription
Abstract Sensitive and robust bioassays for glucocorticoids are very useful for the pharmaceutical industry, environmental scientists and veterinary control. Here, a recombinant yeast cell was constructed that expresses the human glucocorticoid receptor alpha and a green fluorescent reporter protein in response to glucocorticoids. Both the receptor construct and the reporter construct were stably integrated into the yeast genome. The correct and specific functioning of this yeast glucocorticoid bioassay was studied by exposures to cortisol and other related compounds and critically compared to a GR-CALUX bioassay based on a human bone cell. Although less sensitive, the new yeast glucocorticoid bioassay showed sensitivity towards all (gluco)corticoids tested, with the following order in relative potencies: budesonide >> corticosterone > dexamethasone > cortisol = betamethasone > prednisolone > aldosterone. Hormone representatives for other hormone nuclear receptors, like 17ß-estradiol for the oestrogen receptor, 5a-dihydrotestosterone for the androgen receptor and progesterone for the progesterone receptor, showed no clear agonistic responses, whilst some polychlorinated biphenyls were clearly able to interfere with the GR activity.
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