Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 409118
Title Carboxy-terminal extension effects on crystal formation and insecticidal properties of Cry15Aa
Author(s) Naimov, S.; Valkova, R.; Dukjandiev, S.; Minkov, J.; Maagd, R.A. de
Source Journal of Invertebrate Pathology 108 (2011)1. - ISSN 0022-2011 - p. 56 - 8.
Department(s) PRI BIOS Plant Development Systems
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) bacillus-thuringiensis-kurstaki - spodoptera-exigua - domain-iii - protoxin composition - delta-endotoxins - cydia-pomonella - subsp thompsoni - codling moth - proteins - toxicity
Abstract Cry15Aa protein, produced by Bacillus thuringiensis serovar thompsoni HD542, in a crystal together with a 40 kDa accompanying protein, is one of a small group of non-typical, less well-studied members of the Cry family of insecticidal proteins, and may provide an alternative for the more commonly used Cry proteins in insect pest management. In this study we examined the role of the C-terminal part of Cry15Aa and of the 40 kDa protein in crystal formation in recombinant B. thuringiensis. The contribution of the 40 kDa protein and of the Cry15Aa carboxy-terminal sequence for crystal formation, crystal solubilization, and insecticidal properties was assessed. No significant differences in toxicity against Cydia pomonella, before or after in vitro solubilization of crystal-spore preparations, were found. Although the 40 kDa protein significantly contributes to in vitro solubility and in vivo crystal formation of Cry15Aa, no direct evidence for involvement of the 40 kDa protein in toxicity of Cry15Aa was found.
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