Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 409170
Title Bifidobacterium breve - HT-29 cell line interaction: modulation of TNF-a induced gene expression
Author(s) Boesten, R.J.; Vos, W.M. de; Schuren, F.H.J.; Willemsen, L.E.M.; Knol, J.
Source Beneficial Microbes 2 (2011)2. - ISSN 1876-2883 - p. 115 - 128.
Department(s) Microbiological Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) intestinal epithelial-cells - nf-kappa-b - necrosis-factor-alpha - toll-like receptors - immune-response - inflammatory cytokines - dendritic cells - gut flora - in-vitro - lactobacillus
Abstract To provide insight in the molecular basis for intestinal host-microbe interactions, we determined the genome-wide transcriptional response of human intestinal epithelial cells following exposure to cells of Bifidobacterium breve. To select an appropriate test system reflecting inflammatory conditions, the responsiveness to TNF-a was compared in T84, Caco-2 and HT-29 cells. The highest TNF-a response was observed in HT-29 cells and this cell line was selected for exposure to the B. breve strains M-16V, NR246 and UCC2003. After one hour of bacterial pre-incubation followed by two hours of additional TNF-a stimulation, B. breve M-16V (86%), but to a much lesser extent strains NR246 (50%) or UCC2003 (32%), showed a strain-specific reduction of the HT-29 transcriptional response to the inflammatory treatment. The most important functional groups of genes that were transcriptionally suppressed by the presence of B. breve M-16V, were found to be involved in immune regulation and apoptotic processes. About 54% of the TNF-a induced genes were solely suppressed by the presence of B. breve M-16V. These included apoptosis-related cysteine protease caspase 7 (CASP7), interferon regulatory factor 3 (IRF3), amyloid beta (A4) precursor proteinbinding family A member 1 (APBA1), NADPH oxidase (NOX5), and leukemia inhibitory factor receptor (LIFR). The extracellular IL-8 concentration was determined by an immunological assay but did not change significantly, indicating that B. breve M-16V only partially modulates the TNF-a pathway. In conclusion, this study shows that B. breve strains modulate gene expression in HT-29 cells under inflammatory conditions in a strain-specific way
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