Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 409389
Title N-glycoproteomics in plants: Perspectives and challenges
Author(s) Song, W.; Henquet, M.G.L.; Mentink, R.; Dijk, A.D.J. van; Cordewener, J.H.G.; Bosch, H.J.; America, A.H.P.; Krol, A.R. van der
Source Journal of Proteomics 74 (2011)8. - ISSN 1874-3919 - p. 1463 - 1474.
Department(s) Laboratory of Plant Physiology
PRI BIOS Applied Metabolic Systems
PRI BIOS Applied Bioinformatics
PRI BIOS Applied Genomics & Proteomics
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) protein-quality control - capillary-electrophoresis - endoplasmic-reticulum - linked oligosaccharides - mass-spectrometry - oligosaccharyltransferase complex - transmembrane topology - glycosylated proteins - monoclonal-antibody - o-glycosylation
Abstract In eukaryotes, proteins that are secreted into the ER are mostly modified by N-glycans on consensus NxS/T sites. The N-linked glycan subsequently undergoes varying degrees of processing by enzymes which are spatially distributed over the ER and the Golgi apparatus. The post-ER N-glycan processing to complex glycans differs between animals and plants, with consequences for N-glycan and glycopeptide isolation and characterization of plant glycoproteins. Here we describe some recent developments in plant glycoproteomics and illustrate how general and plant specific technologies may be used to address different important biological questions
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