Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 409429
Title Genome constitution of Narcissus variety, 'Tete-a-Tete', analysed through GISH and NBS profiling
Author(s) Wu, H.; Ramanna, M.S.; Arens, P.; Tuyl, J.M. van
Source Euphytica 181 (2011)2. - ISSN 0014-2336 - p. 285 - 292.
Department(s) Laboratory of Plant Breeding
WUR Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) in-situ hybridization - homoeologous recombination - hybrid - origin - chromosomes - lilium - dna
Abstract The Narcissus variety, ‘Tête-à-Tête’, has been the most popular variety since 1949, and a well known allotriploid (2n = 3x = 24 + B) of spontaneous origin. Because the identity of one of the parents of this variety was uncertain, the genome constitution of ‘Tête-à-Tête’ was investigated by using genomic in situ hybridization (GISH) and NBS profiling. Both of these techniques confirmed that two different species of Narcissus, viz., N. tazetta (2n = 2x = 20) and N. cyclamineus (2n = 2x = 14) are the parents. GISH clearly identified 10 chromosomes of N. tazetta and 14 chromosomes of N. cyclamineus, the former has contributed one and the latter has contributed two genomes. One B chromosome was identically labelled as those of N. cyclamineus indicating the affinity of the special chromosome to this species. Due to its male and female sterility ‘Tête-à-Tête’ is unsuitable as a parent for utilizing in further breeding, it might be possible to re-synthesize a ‘Tête-à-Tête’ like variety using the now known parents and the original pathway
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