Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 409489
Title Feasibility of desorption electrospray ionization mass spectrometry for rapid screening of anabolic steroid esters in hair
Author(s) Nielen, M.W.F.; Nijrolder, A.W.J.M.; Hooijerink, H.; Stolker, A.A.M.
Source Analytica Chimica Acta 700 (2011)1-2. - ISSN 0003-2670 - p. 63 - 69.
DOI http://dx.doi.org/10.1016/j.aca.2010.08.009
Department(s) RIKILT - R&C Groeibevorderaars
Laboratory for Organic Chemistry
RIKILT - R&C Diergeneesmiddelen
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) estradiol benzoate - ambient conditions - testosterone - cattle - urine
Abstract Hormone and veterinary drug screening and forensics can benefit from the recent developments in desorption electrospray ionization (DESI) mass spectrometry (MS). In this work the feasibility of DESI application for the rapid screening of intact esters of anabolic steroids in bovine hair has been studied. Using a linear ion trap both full scan and data-dependent collision induced dissociation MSn spectra were acquired in minutes for testosterone cypionate, testosterone decanoate and estradiol benzoate standard solutions deposited on a glass or PTFE surface. However direct analysis of incurred hair failed due to inefficient desorption ionization and the minute quantities of steroid esters present. Therefore a simplified ultrasonic liquid extraction procedure was developed, allowing rapid DESI analysis of a few microliters of the concentrate and a total analysis time of 2–4 h per batch instead of 3 days. The potential of this DESI approach is clearly demonstrated by MS3 data from hair samples incurred with high levels (300–800 µg kg-1) of steroid esters, levels which do occur in samples from controlled- and illegally treated animals. For much lower levels state-of-the-art ultra high performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) screening methods remain the method of choice and might benefit from the proposed simplified extraction as well.
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