Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 409510
Title Protein composition of the occlusion derived virus of Chrysodeixis chalcites nucleopolyhedrovirus
Author(s) Xu, F.; Ince, I.A.; Boeren, S.; Vlak, J.M.; Oers, M.M. van
Source Virus Research 158 (2011)1-2. - ISSN 0168-1702 - p. 1 - 7.
DOI http://dx.doi.org/10.1016/j.virusres.2011.02.014
Department(s) Laboratory of Virology
Biochemistry
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) nuclear polyhedrosis-virus - autographa-californica nucleopolyhedrovirus - per-os infectivity - single nucleocapsid nucleopolyhedrovirus - baculovirus structural protein - dna photolyase genes - open reading frame - multiple nucleopolyhedrovirus - immunocytochemi
Abstract Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) is a group II NPV and its genome has 151 predicted open reading frames. In this study, the protein composition of ChchNPV occlusion derived virus (ODV) was determined by LC–MS/MS. Fifty-three proteins were identified in ChchNPV ODV particles. One ODV-protein is encoded by a gene so far unique to ChchNPV (Chch105). The two DNA photolyases PHR1 and PHR2, which are characteristic for ChchNPV and thought to be involved in repairing UV damage in viral DNA, were not detected in the ODVs. Comparison of the ODV proteins identified in ChchNPV and in three other baculoviruses enabled the identification of ten conserved ODV proteins (ODV-E18, ODV-E56, ODV-EC27, ODV-EC43, P6.9, P33, P49, P74, GP41, and VP39). In addition, the baculovirus per os infectivity factors PIF1, PIF2 and PIF3 were all detected in ChchNPV and these should be considered as conserved ODV proteins as well as they are absolutely required for oral infection. With the LC–MS/MS method used 22 viral proteins were detected, which were not identified as ODV proteins in previous studies.
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