Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 409578
Title Induced point mutations in the phytoene synthase 1 gene cause differences in carotenoid content during tomato fruit ripening
Author(s) Gady, A.L.F.; Vriezen, W.; Wal, M.H.B.J. van de; Huang, P.; Bovy, A.G.; Visser, R.G.F.; Bachem, C.W.B.
Source Molecular Breeding 29 (2012)3. - ISSN 1380-3743 - p. 801 - 812.
Department(s) Laboratory of Plant Breeding
WUR Plant Breeding
PRI BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) biosynthesis - expression - lycopene - cloning - plants
Abstract In tomato, carotenoids are important with regard to major breeding traits such as fruit colour and human health. The enzyme phytoene synthase (PSY1) directs metabolic flux towards carotenoid synthesis. Through TILLING (Targeting Induced Local Lesions IN Genomes), we have identified two point mutations in the Psy1 gene. The first mutation is a knockout allele (W180*) and the second mutation leads to an amino acid substitution (P192L). Plants carrying the Psy1 knockout allele show fruit with a yellow flesh colour similar to the r, r mutant, with no further change in colour during ripening. In the line with P192L substitution, fruit remain yellow until 3 days post-breaker and eventually turn red. Metabolite profiling verified the absence of carotenoids in the W180* line and thereby confirms that PSY1 is the only enzyme introducing substrate into the carotenoid pathway in ripening fruit. More subtle effects on carotenoid accumulation were observed in the P192L line with a delay in lycopene and b-carotene accumulation clearly linked to a very slow synthesis of phytoene. The observation of lutein degradation with ripening in both lines showed that lutein and its precursors are still synthesised in ripening fruit. Gene expression analysis of key genes involved in
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