Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 409579
Title Isolation and characterisation of a strawberry fruit-specific promotor
Author(s) Schaart, J.G.; Salentijn, E.M.J.; Pelgrom, K.T.B.; Aharoni, A.; Krens, F.A.
Source Genes, Genomes and Genomics 5 (2011)Special Issue 1. - ISSN 1749-0383 - p. 108 - 114.
Department(s) WUR Plant Breeding
PRI Bioscience
Publication type Refereed Article in a scientific journal
Publication year 2011
Abstract In order to achieve specific expression of transgenes in strawberry fruits, the availability of tissue- (receptacle) specific promoter sequences is desired. For this reason, 5'-upstream sequences of the strawberry expansin gene FaExp2, which is expressed in a fruit-specific manner, have been isolated. To characterise the promoter activity of the isolated sequences, fragments of 0.7 kb (0.7pFaExp2)and 1.6 kb (1.6pFaExp2) have been fused to the ß-glucuronidase reporter gene (gus). In transgenic strawberry plants transformed with either 0.7pFaExp2-gus or1.6pFaExp2-gus, a fruit-specific expression pattern was observed for both promoter constructs. However, quantitative RT-PCR revealed that gus expression levels driven by the 1.6pFaExp2 promoter fragment were much more higher. In addition to the expression in fruits, both promoter fragments also seemed to direct gene expression in the achenes and to some extent in epidermal and subepidermal tissues of petioles and stems of flowers and fruits. It is concluded that both promoter sequences are suitable for directing transgene expression in strawberry fruits in a specific way
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