Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 410022
Title Microarray-Based Identification of Transcription Factor Target Genes
Author(s) Gorte, M.; Horstman, A.; Page, R.B.; Heidstra, R.; Stromberg, A.; Boutilier, K.A.
Source In: Plant Transcription Factors: Methods and Protocols. / Yuan, L., Perry, S.E., Humana Press (Methods in Molecular Biology 754) - ISBN 9781617791536 - p. 119 - 141.
Department(s) Laboratory of Molecular Biology
PRI BIOS Plant Development Systems
Publication type Peer reviewed book chapter
Publication year 2011
Abstract Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.
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